11/3/2022 0 Comments Capto q resin![]() ![]() 1.2 min residence ( ■) 2.4 min residence ( ■). Thyroglobulin (TGY) solution (1.1 mg/ml) in 20 mM Tris Cl, pH 8.0 was loaded onto the columns until 10% breakthrough was observed. The resins were packed into 1 ml columns. Comparison of DBCs between Nuvia HP-Q and three commercially available resins at 1.2 and 2.4 min residence times. Parallel runs of thyroglobulin on Nuvia HP-Q and other commercially available resins demonstrate that Nuvia HP-Q shows best-in-class DBC and overcomes the compromised productivity issue that results from low DBCs of other IEX resins (Figure 2).įig. Resinģ Data obtained from vendor presentation. Superior DBC of Nuvia HP-Q at a high flow rate relative to other comercially available resins. In contrast, other resins showed lower DBC or achieved the same DBC at a much lower flow rate (Table 1). IgM obtained from plasma fractionation showed DBC in the range of 20–25 mg/ml with Nuvia HP-Q. Nuvia HP-Q is designed to overcome the issues faced when purifying large biomolecules with other commercially available resins and help in downstream purification of such biomolecules at fast flow rates without loss in DBC and recovery. In large biomolecule purification, how does Nuvia HP-Q compare to other commercially available resins? Easy accessibility of large molecules for binding. Traditional ion exchange (IEX) resins don’t offer this advantage.įig. Its pore size is optimized for easy accessibility and adsorption of large biomolecules, and the internal spacer length and ligand density facilitate efficient binding of the biomolecules even at high flow rates (Figure 1). High Target Recovery Why will you achieve high target recovery with Nuvia HP-Q? ![]() It’s robust and stable base bead provides the high-throughput purification (HTP) demanded by today’s process manufacturing. Its optimized particle and pore size offers high dynamic binding capacity (DBC) at fast flow rates without excessive backpressure and fast mass transfer kinetics, thereby delivering excellent target recovery and improved process economics. Nuvia HP-Q is a high-performance strong anion exchange resin designed for purification of large biomolecules such as plasma proteins, IgA and IgM, viruses, VLPs, and PEGylated proteins. ![]()
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